Figure 3.
FTO knockdown upregulated inflammation and promoted microglial migration. (A,B) RT-qPCR and Western blot for determining FTO knockdown efficiency (n = 3, unpaired Student's t-test). (C) Percentage of m6A content of the total RNA in the vehicle group and the shFTO group (n = 3, unpaired Student's t-test). (D) CCK-8 assay for determining the proliferation of the vehicle and shFTO groups (n = 3; unpaired Student's t-test). (E, F) The mRNA and protein levels of iNOS, TNFα, and IL6 in the vehicle- and shFTO-treated cells with or without LPS + IFN-γ stimulation (n = 3; one-way ANOVA). (G) ELISAs for testing TNFα expression in the vehicle- or shFTO-treated cells with or without LPS + IFN-γ stimulation (n = 3; one-way ANOVA). (H) Transwell images and corresponding quantifications of the vehicle group, vehicle + LPS&IFN-γ group, shFTO group, and shFTO + LPS&IFN-γ group (n = 3; one-way ANOVA). (I) Representative images of the wound-healing assay performed in the vehicle group, vehicle + LPS&IFN-γ group, shFTO group, and shFTO + LPS&IFN-γ group (n = 3; one-way ANOVA). ∗P < 0.05, ∗∗P < 0.01.