Figure 4.

GPC4 might be the downstream target of FTO. (A,B) The volcano plot of DEGs in the vehicle + LPS&IFN-γ and shFTO + LPS&IFN-γ cells (| log₂FC | > 1, Padj < 0.05). (C) The heatmap of significant DEGs in the abovementioned groups (| log₂FC | > 1, Padj < 0.05). (D,E) The RT-qPCR and Western blot for validating GPC4 expression in the abovementioned groups. (F) m⁶A enrichment of GPC4 mRNA was detected by MeRIP-qPCR in the abovementioned groups. (G) RT-qPCR for showing GPC4 mRNA expression after the administration of ActD (5 μg/mL) for 0, 2, 4, and 6 h in the abovementioned groups. (H) RT-qPCR for detecting GPC4 mRNA levels in the shFTO + siYTHDF3 group and the shFTO + vector group. (I) RNA stability analysis in the shFTO + siYTHDF3 group and shFTO + vector group (n = 3). Values are analyzed using the unpaired Student's t-test. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.