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. 2022 Oct 4;10(5):2179–2193. doi: 10.1016/j.gendis.2022.09.008

Figure 5.

Figure 5

FTO-mediated GPC4 regulated microglia through the TLR4/NF-κB signaling pathway. (A) Identification of the most significant module from the PPI network using the STRING database. (B) KEGG pathway enrichment analysis of DEGs. (C) Schematic diagram of the conjectured pathway. (D) The mRNA levels of CXCL9, CXCL10, CXCL11, CXCR3, TLR4, and CD14 in the vehicle + LPS&IFN-γ group, and the shFTO + LPS&IFN-γ group were measured via RT-qPCR. (E) Western blotting for showing the protein expression of NF-κB p-p65 (S536), p65, TLR4, MYD88, and CD14 in the vehicle + LPS&IFN-γ group and the shFTO + LPS&IFN-γ group (n = 3). Values are analyzed using the unpaired Student's t-test. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.