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. 2022 Oct 4;10(5):2179–2193. doi: 10.1016/j.gendis.2022.09.008

Figure 7.

Figure 7

FTO inhibition aggravated EAU progression in vivo. (A) Retinal FTO mRNA and protein expression in the FB23-2-treated mice and the PBS + DMSO-treated mice (n = 3). (B) Experimental flowchart describing the modeling process and drug injection. (C) Representative images and quantification of the ocular anterior chamber (upper) and eyeball sections (lower) (n = 5). Black arrows indicate inflammatory cells. Red arrows indicate conjunctival or ciliary hyperemia, or vasculitis. White arrows indicate posterior synechiae, or retinal folds. Scale bar, 100 μm. (D, E) RT-qPCR and Western blot for showing the expression levels of iNOS, TNFα, and IL6 in the EAU + vehicle and EAU + FB23-2 groups (n = 3). (F) Protein expression levels of GPC4, TLR4, p-p65, and p65 in the EAU + vehicle and EAU + FB23-2 groups (n = 3). Values are analyzed using the unpaired Student's t-test. ∗P < 0.05; ∗∗P < 0.01. (G) Graphical summary of this study.