FIG. 5.

Effect on growth of inactivation of asnB, asnH, asnO, and ytnA in B. subtilis. A single colony each of strains 168 (wild type [WT]), FU340 (ΔB), FU341 (ΔH), FU342 (ΔO), FU343 (ΔBH), FU344 (ΔBO), FU345 (ΔHO), and FU346 (ΔBHO) was taken from overnight cultures on TBABG plates containing appropriate antibiotics and spread onto S6 plates with (right) and without (left) asparagine (50 μg/ml). The plates were incubated at 37°C for 36 h, and then colony formation was observed. Data from a single experiment are presented. The same experiments were repeated three times independently with similar results.