TABLE 1.
Bacterial strains used in this study
| Strain | Genotype | Source or referencea |
|---|---|---|
| B. subtilis | ||
| 168 | trpC2 | Our standard strain |
| BFS41 | trpC2 ytnA::pMUTIN2mcs | This work (pYTNA1 → 168) |
| BFS55 | trpC2 asnH::pMUTIN2mcs | This work (pASNH1 → 168) |
| BFS56 | trpC2 PasnB::pMUTIN2mcs | This work (pASNB5D → 168) |
| FU339 | trpC2 PasnO::pMUTIN2mcs | This work (pMASNO → 168) |
| FU340 | trpC2 ΔasnB::neo | This work |
| FU341 | trpC2 ΔasnH::spc | This work |
| FU342 | trpC2 ΔasnO::cat | This work |
| FU343 | trpC2 ΔasnB::neo ΔasnH::spc | This work |
| FU344 | trpC2 ΔasnB::neo ΔasnO::cat | This work |
| FU345 | trpC2 ΔasnH::spc ΔasnO::cat | This work |
| FU346 | trpC2 ΔasnB::neo ΔasnH::spc ΔasnO::cat | This work |
| FU347 | trpC2 ytnA::pMUTIN2mcs ΔasnH::spc | This work |
| FU348 | trpC2 ytnA::pMUTIN2mcs ΔasnH::spc ΔasnO::cat | This work |
| ASK201 | trpC2 spoOH::erm | Kei Asai (spoOH::erm → 168) |
| ASK202 | trpC2 spoIIAC::kan | Kei Asai (spoIIAC::kan → 168) |
| ASK203 | trpC2 spoIIGAB::kan | Kei Asai (spoIIGAB::kan → 168) |
| ASK204 | trpC2 spoIIIG::kan | Kei Asai (spoIIIG::kan → 168) |
| ASK205 | trpC2 spoIVCB::erm | Kei Asai (spoIVCB::erm → 168) |
| E. coli | ||
| JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′[traD36 proAB+ lacIqlacZΔM15] | 23 |
| ME6279 | F−asnA asnB thi thy str recA | 13 |
| C600 | supE44 hsdR thi-1 thr-1 leuB6 lacY1 tonA21 | 24 |
a
Arrows indicate transformation from donor DNA to recipient strain. Strain 168 is the strain chosen for the international B. subtilis genome functional analysis project. Strains ASK201 to -205 were kind gifts from Kei Asai (Nara Institute of Science and Technology, Ikoma, Japan); each of the mutations of sigma factor genes was transferred to strain 168 from the original mutant strains established by him or by Patrick Stragier (Institut de Biologie Physico-Chimique, Paris, France). The original strains are MO1781, MO719, MO718, UOT-1850 (1), and MO1027, for the sigma-E (spoIIGAB::kan), -F (spoIIAC::kan), -G (spoIIIG::kan), -H (spoOH::erm), and -K (spoIVCB::erm) mutations, respectively.