FIGURE 1.

Schematic representation of cryptochrome- and improved light-inducible dimer (iLID)-based optogenetics. (A) Schematic illustrating recruitment of cytosolic CRY2PHR-mCherry fused with a protein of interest to the plasma membrane anchor, CIBN-CAAX, by global or local illumination with 488 nm laser. (B) Schematic demonstrating recruitment of cytosolic SspB-RFP fused with a protein of interest to the membrane anchor, iLID-CAAX, by global or local illumination with 488 nm light. (C) Depending on the region of the plasma membrane where 488 nm light was applied, CRY2PHR or SspB was recruited either all over the cell boundary (global recruitment), or specifically to the front or back of the cell (local recruitment).