Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 10;11:1195806. doi: 10.3389/fcell.2023.1195806

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Pal, Lin, Zhan, Banerjee, Kuhn, Providence and Devreotes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Establishment of LOV domain-based iLID system in macrophages and Dictyostelium. (A) Time-lapse confocal images of RAW 264.7 macrophage expressing tgRFPt-SSPB R73Q (or sspb) before or after 488 nm laser was turned on globally. Time in min:sec format. Scale bars represent 5 μm. (B) Time-lapse images of RAW 264.7 macrophage expressing tgRFPt-SSPB R73Q (or sspb) which was recruited exclusively to one side of the cell by applying 488 nm light near it, as denoted by dashed white box. Both (A, B) highlight the fast reversibility of this system. Time in min:sec format. Scale bars represent 5 μm. (C) Time-lapse confocal images of a field of vegetative Dictyostelium expressing tgRFPt-SSPB R73Q (or sspb) before or after 488 nm laser was switched on globally. Pink arrows denote successful recruitment in cells. Time in min:sec format. Scale bars represent 5 μm. (D) Time-lapse images of vegetative Dictyostelium expressing tgRFPt-SSPB R73Q (or sspb) which was recruited exclusively to one side of the cell by applying 488 nm light near it, as denoted by dashed white box. Time in min:sec format. Scale bars represent 5 μm. (E) A linescan across the cytosol-membrane of the cell in (D) denoting increased sspb intensity on the membrane after laser was switched on near the region. (F) Schematic representation of experimental data shown in (A). (G) Schematic representation of experimental data shown in (B, D). Both (F, G) highlight fast reversibility of iLID system with both global or local recruitment experiments.