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Basal expression of Dlx5 and Col10a1 in ATDC5 cells. (A, B) ATDC5 cells were induced with a complete medium supplemented with 1 × ITS for the corresponding number of days. The mRNA level of Col10a1, Dlx5, and Runx2 were measured by qRT-PCR. (C)Western blotting results showing the protein level of COL10A1, DLX5, and RUNX2 after 14-day ITS induction. Data were expressed as mean ± SEM from three independent experiments, ∗P < 0.05.
