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. 2023 Feb 1;10(5):2097–2108. doi: 10.1016/j.gendis.2022.12.016

Figure 3.

Fig. 3

Dlx5 promotes the expression of Col10a1 in MCT cells. (A) The mRNA levels of Dlx5 in MCT cells after transfected with pcDNA3.1/Dlx5 and pcDNA3.1 for 24 h. (B) The mRNA levels of Col10a1 after transfected with pcDNA3.1/Dlx5 and pcDNA3.1 for 24 h. (C) Western blotting analysis was used to examine the protein expression of DLX5 and COL10A1 in MCT cells transiently transfected with pcDNA3.1/Dlx5 and pcDNA3.1 for 48 h. β-actin was used as an internal control. (D) The mRNA levels of Dlx5 in MCT cells transfected with Dlx5 siRNA-A and scrambled sequences for 24 h. (E) The mRNA levels of Col10a1 in MCT cells transfected with Dlx5 siRNA-A and scrambled sequences for 24 h. (F) Western blotting analysis was used to examine the protein expression of DLX5 and COL10A1 in MCT cells transiently transfected with Dlx5 siRNA-A and scrambled sequences for 48 h. β-actin was used as an internal control. The results support that forced expression of Dlx5 further increases, while knockdown of Dlx5 decreases Col10a1 expression in hypertrophic MCT cells. Data were expressed as mean ± SEM from three independent experiments. ∗P < 0.05, ∗∗P < 0.01.