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. 2023 Feb 1;10(5):2097–2108. doi: 10.1016/j.gendis.2022.12.016

Figure 4.

Fig. 4

DLX5 strengthens the activity of the Col10a1 enhancer. (A) 293T cells were transiently transfected with pcDNA3.1/Dlx5 and pcDNA3.1 for 24 h. The relative mRNA level of Dlx5 was detected by qRT-PCR. (B) Western blotting analysis was used to examine the protein expression of DLX5 in 293 T cells transiently transfected with pcDNA3.1/Dlx5 and pcDNA3.1 for 48 h. β-actin was used as an internal control. (C) Dual-Luciferase reporter gene assays were performed to detect relative fluorescence activity in 293T cells. The results demonstrated that DLX5 can promote the activity of the Col10a1 enhancer. Data were expressed as mean ± SEM from three independent experiments. ∗P < 0.05, ∗∗P < 0.01.