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DLX5 can directly bind to the 150-bp Col10a1 cis-enhancer. MCT cells cultured at 32 °C (proliferative) (A) and 37 °C (hypertrophy) (B) were subjected to chromatin immunoprecipitation (ChIP) assays and the results demonstrated higher recruitment of DLX5 to the Col10a1 enhancer both in proliferative and in hypertrophic chondrocytes, indicating a direct interaction between DLX5 and Col10a1 ∗∗P < 0.01, ∗∗∗P < 0.001.
