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. 2023 Feb 1;10(5):2097–2108. doi: 10.1016/j.gendis.2022.12.016

Figure 6.

Fig. 6

Histology and immunohistochemistry analyses of COL10A1 and DLX5 expression in OA mice. (A) The mRNA levels of Col10a1 and Dlx5 in the sham and DMM group. Six weeks after DMM surgery, articular cartilage and part of the subchondral bone tissues were quickly collected and snap-frozen in liquid nitrogen, grounded into powder, and RNA was extracted following the TRizol instructions. As shown in Figure 6A, the mRNA levels of Dlx5 and Col10a1 in articular cartilage increased significantly six weeks after surgery. (B) The hematoxylin and eosin, safranin O-fast green, and immunohistochemistry staining of COL10A1 and DLX5 in the sham and DMM group. Frontal sections of the knee joints were taken and subjected to hematoxylin and eosin and safranin O-fast green staining, smooth and intact knee joint structure could be seen in sham control, while OA mice showed rough surface of the knee joints and joint space narrowing with increased hypertrophic chondrocytes (left two panels). Magnification = 400×. Scale bar = 50 μm. Meanwhile, COL10A1 and DLX5 proteins were stained brown dots after DLX5 and COL10A1 immunostaining in mouse surgery-induced OA cartilage lesions and sham group. Immunohistochemistry staining showed increased hypertrophic chondrocytes with significantly up-regulated COL10A1 and DLX5 expression in the OA mice compared with the sham control (right two panels). Magnification = 400 ×. Scale bar = 50 μm.