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. 2022 Nov 23;10(5):2082–2096. doi: 10.1016/j.gendis.2022.10.021

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© 2022 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Low expression of LRP1B in hepatocellular carcinoma is associated with cell migration, proliferation, and apoptosis. (A)LRP1B expression was measured in adjacent and tumor tissues. (B) Relative mRNA expression of LRP1B in different cell lines. (C) Immunofluorescence staining for LRP1B. (D) EdU incorporation assay. (E) Scratch assay of Huh7 cells. (F) Plate colony formation assay. (G) Transwell migration assay. (H) Apoptosis was analyzed by flow cytometry. (I) The cell cycle distribution was analyzed by flow cytometry.