Table 3.
Results of metagenomic analysis.
| Sample ID | Sample origin | DNA concentration (ng/μL) | Total reads | Human genome reads | Human genome (%) | Remaining reads after exclusion of human genome | Number of reads for E. coli (rate of total reads) |
|---|---|---|---|---|---|---|---|
| P1 | Small intestine | 49.0 | 17,154,163 | 16,985,393 | 99.0 | 168,770 | 178 (1.04 × 10−5) |
| P2 | Duodenum | 60.0 | 24,030,443 | 23,928,673 | 99.6 | 101,770 | 1 (4.16 × 10−8) |
| P3 | Jejunum | 25.0 | 15,064,268 | 14,758,401 | 98.0 | 305,867 | 141 (9.36 × 10−6) |
| P4 | Ileum | 33.8 | 13,208,642 | 13,120,565 | 99.3 | 88,077 | 15 (1.14 × 10−6) |
| P5 | Bile duct | 0.8 | 860,450 | 505,987 | 58.8 | 354,463 | 522 (6.07 × 10−4) |
| P6 | Pancreas | 1.3 | 915,292 | 14,028 | 1.5 | 901,264 | 18 (1.97 × 10−5) |
| P7a | Jejunum | 10.9 | 9,829,707 | 9,082,885 | 92.4 | 746,822 | 95,875 (9.75 × 10−3) |
| P8 | Left colon | 0.1 | 9,645 | 9,331 | 96.7 | 314 | 0 |
| P9 | Left colon | 27.5 | 23,918,080 | 23,401,541 | 97.8 | 516,539 | 21 (8.78 × 10−7) |
| P10 | Gallbladder | 27.2 | 18,710,915 | 18,626,399 | 99.5 | 84,516 | 12 (6.41 × 10−7) |
E. coli was detected in only P7 by the multiplex PCR system.