Table 4.
Results of culture and the multiplex PCR system with metagenomic analysis.
| Sample ID | Culture-positive microorganism | Number of reads in the metagenomic analysis | Rate of reads in the metagenomic analysis | Multiplex PCR system-positive microorganism | Number of reads in the metagenomic analysis | Rate of reads in the metagenomic analysis |
|---|---|---|---|---|---|---|
| P1 | Streptococcus anginosus (2+) | 3,723 | 2.17 × 10−4 | Streptococcus | 6,796 | 3.96 × 10−4 |
| P1 | E. coli (small amount) | 178 | 1.04 × 10−5 | |||
| P2 | Cadida albicans (small amount) | 1,905 | 7.93 × 10−5 | Candida albicans | 1,905 | 7.93 × 10−5 |
| P3 | Enterococcus faecium (1+) | 63,060 | 4.19 × 10−3 | Enterococcus | 83,555 | 5.55 × 10−3 |
| P3 | Klebsiella aerogenes (1+) | 23,065 | 1.53 × 10−3 | Enterobacteriaceae | 25,387 | 1.69 × 10−3 |
| P3 | Citrobacter freundii (small amount) | 57 | 3.78 × 10−6 | Streptococcus | 19,500 | 1.29 × 10−3 |
| P3 | Candida albicans | 214 | 1.42 × 10−5 | |||
| P4 | Klebsiella pneumoniae (1 colony) | 19 | 1.44 × 10−6 | Klebsiella pneumoniae | 19 | 1.44 × 10−6 |
| P4 | Enterobacteriaceae | 111 | 8.40 × 10−6 | |||
| P4 | Candida glabrata | 33,336 | 2.52 × 10−3 | |||
| P5 | Klebsiella pneumoniae (2+) | 2,006 | 2.33 × 10−3 | Klebsiella pneumoniae | 2,006 | 2.33 × 10−3 |
| P5 | Pseudomonas aeruginosa(2+) | 1,446 | 1.69 × 10−3 | Pseudomonas aeruginosa | 1,446 | 1.69 × 10−3 |
| P5 | Citrocbactor freundii(2+) | 20,851 | 2.42 × 10−2 | Enterobacteriaceae | 101,143 | 1.18 × 10−1 |
| P5 | Enterococcus faecium (2+) | 40,149 | 4.67 × 10−2 | Enterococcus | 42,463 | 4.93 × 10−2 |
| P5 | Candida tropicalis (small amount) | 36 | 4.18 × 10−5 | Candida tropicalis | 36 | 4.18 × 10−5 |
| P5 | Klebsiella oxytoca | 29,816 | 3.47 × 10−2 | |||
| P5 | Streptococcus | 83 | 9.65 × 10−5 | |||
| P6 | Streptococcus anginosus (1+) | 2,945 | 3.22 × 10−3 | Streptococcus | 6,812 | 7.44 × 10−3 |
| P6 | Serratia marcescens (3 colonies) | 589 | 6.44 × 10−4 | Serratia marcescens | 589 | 6.44 × 10−4 |
| P6 | Staphylococcus aureus:MRSA (1 colony) | 17 | 1.86 × 10−5 | Staphylococcus | 531 | 5.80 × 10−4 |
| P6 | Prevotella, porphyromonas (3+)a | 22,634 | 3.51 × 10−4 | mecA(methicillin-resistancegene) | ||
| P6 | Fusobacterium nucleatum (3+)a | 42,998 | 2.50 × 10−4 | Enterobacteriaceae | 67 | 7.32 × 10−5 |
| P6 | Peptostreptococcus micros (3+)a | 229 | 4.70 × 10−2 | Candida albicans | 0 | |
| P7 | Escherichia coli (3+) | 95,875 | 9.75 × 10−3 | Escherichia coli | 95,875 | 9.75 × 10−3 |
| P7 | Klebsiella pneumoniae (3+) | 5,455 | 5.55 × 10−4 | Enterobacteriaceae | 114,365 | 1.16 × 10−2 |
| P7 | Bacteroides fragilis group (2+)a | 8,157 | 8.30 × 10−4 | Klebsiella pneumoniae | 5,455 | 5.55 × 10−4 |
| P7 | Eubacterium sp. (2+)a | 208 | 2.12 × 10−5 | Klebsiella oxytoca | 2,102 | 2.14 × 10−4 |
| P7 | Morganella morganii (small amount)a | 295 | 3.00 × 10−5 | Streptococcus | 562 | 5.72 × 10−5 |
| P7 | Candida albicans | 5 | 5.09 × 10−7 | |||
| P7 | Candida glabrata | 28 | 2.85 × 10−6 | |||
| P8 | Enterococcus faecalis (1+) | 249 | 2.58 × 10−2 | Enterococcus | 254 | 2.63 × 10−2 |
| P8 | Enterococcus avium (1+) | 2 | 2.07 × 10−4 | Candida albicans | 0 | 0 |
| P8 | E.coli (after 24 h enrichment) | 0 | 0 | |||
| P9 | Prevotella sp. (2+)a | 89 | 3.72 × 10−6 | Streptococcus | 772 | 3.23 × 10−5 |
| P10 | Citrobacter freundii (few colonies) | 77 | 4.12 × 10−6 | Enterobacteriaceae | 2,400 | 1.28 × 10−4 |
a
Microorganisms not included in the blood culture identification panel.