Figure 1.

Liposarcoma maturity score anticorrelates with MDM2 levels

(A) Schematic of study design with indication of primary tumors and cell line models (left) and genome-wide assays (right).

(B) Plot depicts copy number alterations in normal fat (gray), WDLPS (red), and DDLPS (blue) samples from our clinical cohort. Vertical plot at left depicts the average copy number across the genome, while the horizontal view expands the Chr12q locus that contains MDM2.

(C) Box plot shows distribution of MDM2 expression in normal fat and WDLPS and DDLPS. One-tailed t test p values: DDLPS vs. WDLPS = 0.09, NF vs. WDLPS = 8.8e−5, NF vs. DDLPS = 5.5e−5.

(D) Waterfall plots show maturity scores for samples in the clinical cohort.

(E) Hematoxylin and eosin staining for representative well-differentiated (WD) and dedifferentiated (DD) tumors, ranked by maturity score (top) and showing expression of master adipocytic regulators PPARG and CEBPA (bottom).

(F) Scatterplots show correlation between MDM2 expression (y axis) and maturity score (x axis) for WDLPS and DDLPS tumors.