Supraphysiologic MDM2 localizes to nuclear foci
(A) IF images (Airyscan confocal, 63×) for representative LPS853 cell show MDM2 (green), P53 (red), and DAPI (blue).
(B) IF images (confocal, 63×) for representative LPS853 cell show MDM2 (green), PML (red), and DAPI (blue).
(C) Quantification of MDM2 and PML intensity profiles across white dashed line in (B) shows co-localization of MDM2 and PML.
(D and F) IF images (confocal, 63×) for LPS853 cells show MDM2 (green), YY1 (red), and DAPI (blue).
(E and G) High-magnification images shown for white boxed regions in (D) and (F). Bottom plots show quantifications of MDM2 (green) and YY1 (red) intensities across white dashed lines.