Figure 2.

AcALMT1 functions as a citrate transporter. (a) AcALMT1 mediated citrate transport in Xenopus laevis oocyte system. Whole‐cell current recorded in X. laevis oocytes injected with H₂O (control) or AcALMT1‐cRNA. The bath solution was added with citrate at a final concentration of 20 mm. The holding potential ranged from 40 to −20 mV in 10 mV increments. The time and current scale bars for the recordings are shown. (b) The current–voltage (I–V) relationship of the steady state currents were recorded in oocytes injected with H₂O (control) or AcALMT1‐cRNA. Data are shown as means ± SE (n = 6). (c) A significantly elevated level of citrate content in kiwifruit core tissues by transiently over‐expressing (TOE) AcALMT1. Fruit cores transiently transformed with the empty vector were used as controls (EV). Error bars indicate SE from four biological replicates (**P < 0.01).