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. 2023 May 17;21(8):1707–1715. doi: 10.1111/pbi.14071

Figure 1.

Figure 1

Development and characterization of maize RUBY haploid inducer. (a) A schematic of RUBY expression construct in maize. Three betalain biosynthetic genes CYP76AD1, DODA and Glucosyltransferase were linked with ‘self‐cleaving’ 2A peptides and driven by ZmUbi promoter. pZmUbi, ZmUbi promoter; P2A, ‘self‐cleaving’ 2A peptides; tE9, E9 terminator; p35S, CaMV 35S promoter; t35S, CaMV 35S terminator. (b–g) Pigmentation of plant (b), immature embryos (c), germinated embryos (d, e), ear (f) and seedling (g) of RUBY haploid inducer. (h, i) The mature seeds of haploid and diploid from ZD958 × RUBY haploid inducer (h) and CHOI4 (i), respectively. Scale bars, b, 10 cm; c‐e, 0.5 cm; f and g, 3 cm; h and i, 1 cm.