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. 2023 May 17;21(8):1707–1715. doi: 10.1111/pbi.14071

Figure 4.

Figure 4

Development and characterization of tomato RUBY haploid inducer. (a) A schematic of RUBY expression vector in tomato. p35S, CaMV 35S promoter; P2A, ‘self‐cleaving’ 2A peptides; t35S, CaMV 35S terminator; pNOS, Nopaline synthase promoter; tOCS, Octopine synthase terminator. (b) A candidate tomato RUBY haploid inducer plant. (c) The bottom, middle and top leaves of RUBY haploid inducer. (d) The flowers of RUBY haploid inducer (up) and Alisa Craig (down). (e, f) The fruits of RUBY inducer and Alisa Craig at the green (left), pink (middle) and red (right) stages of ripening. (g–i) RUBY functions in tomato seeds and radicles. (j) The seedling of RUBY haploid inducer with red cotyledons and hypocotyl. (k–m) The seeds and radicles of Alisa Craig. (n) The seedling of Alisa Craig with green cotyledons and white hypocotyl. Scale bars, b and c, 10 cm; d, g–n, 1 cm; e and f, 4 cm.