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. 2023 May 12;21(8):1682–1694. doi: 10.1111/pbi.14069

Figure 3.

Figure 3

Expression pattern of TOE4b and subcellular localization of TOE4b in soybean. (a) Expression pattern of TOE4b. The plants were grown under SD (12‐h light/12‐h dark) conditions in a plant growth chamber. (b, c) Diurnal expression pattern of TOE4b in leaves under LD (16‐h light/8‐h dark) (b) and SD (12‐h light/12‐h dark) conditions (c). Three leaves were collected from different seedlings at 18 DAE. β‐Tubulin (TUB) was used as a reference transcript. The cultivar Williams 82 (W82) was used. (d) Schematic diagrams of the constructs used for subcellular localization. (e) TOE4b localizes to the nucleus. The vector control (35S:GFP) and 35S:TOE4b‐GFP were introduced into Nicotiana benthamiana leaves via Agrobacterium‐mediated infiltration. Panels from left to right show the bright field, GFP signal channel, the DAPI stain and the merged images. Scale bars, 20 μm.