Figure 4.

Phenotypes of TOE4b overexpression lines and mutant. (a‐f) Phenotypes of TOE4b overexpression lines. Flowering time (b, e). Average internode length (c, f), calculated as the plant height divided by the number of nodes. The plants were grown in an incubator with an average photon flux of 300 μmol m⁻² s⁻¹ under LD (16‐h light/8‐h dark) (a‐c) or SD (12‐h light/12‐h dark) (d‐f) conditions. Phenotypes were recorded at 44 DAE for LD treatment and at 25 DAE for SD treatment. (g‐i) Phenotypes of toe4b mutant under LD (16‐h light/8‐h dark) conditions. The plants were grown in an incubator with an average photon flux of 450 μmol m⁻² s⁻¹. W82, wild‐type Williams 82. The red boxes are enlarged to the right and show the axils of trifoliate leaves. Data are means ± SD, n = 5. P < 0.05, as determined by multiple comparison testing by one‐way ANOVA. Different lowercase letters indicate significant differences. (j) Schematic diagram of TOE4b and the selected target site for CRISPR/Cas9‐mediated gene editing. (k) Sequence alignment of the single‐guide RNA (sgRNA) target region in W82 and the homozygous mutant. Red dashes indicate deleted nucleotides. (l) Predicted changes in the amino acid sequence of TOE4b from wild‐type W82 and the toe4b mutant. Underlined nucleotides, sgRNA target sites; red boxes, protospacer‐adjacent motif (PAM); blue letters show the amino acids that are different from W82; asterisk, termination of translation.