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. 2023 May 18;3(7):100329. doi: 10.1016/j.xgen.2023.100329

Figure 1.

Figure 1

Proteomics approach for SUMO site detection

(A) Diagram of the peptides generated after trypsin digestion of proteins modified by wild-type and T > R mutant SUMO.

(B) Diagram of the experimental design and work flow for SUMOylation-derived diGly-modified peptide MS analysis.

(C) Diagram of the recombinant SUMO construct used to generate SUMO-TR expressing transgenic flies.

(D) Western blot of total ovary lysate confirming the expression of recombinant SUMO. Ovaries from flies overexpressing 6xHis-FLAG-tagged SUMO are used as a positive control.