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. 2023 May 18;3(7):100329. doi: 10.1016/j.xgen.2023.100329

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© 2023 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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SUMO targets nuclear and cytoplasmic piRNA factors

(A) Network diagram showing physical interacting partners of Piwi, HP1, SetDB1, Wde, and Vasa proteins retrieved from FlyBase. Nodes are colored according to detection frequency of SUMOylation sites in the MS data. Additional interactions between partner nodes are not shown.

(B) (Top) Diagram of an ovariole with indicated stages where the maternal tubulin-Gal4 (MT-gal4) driver is active; stages of imaging are highlighted. (Bottom) Confocal images of Vasa (immunostaining) and Aub (GFP-tagged Aub) localization in stage 5–7 egg chambers from flies expressing SUMO shRNA under the control of the MT-gal4 driver, or no-driver control siblings. Scale bar, 20 μm.