REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-Flag (HRP conjugated)	Sigma	Cat# A8592; RRID:AB_439702
Rabbit polyclonal anti-GFP	Abcam	Cat# ab290; RRID:AB_303395
Rabbit polyclonal anti-GFP	Chen et al., 201647	N/A
Anti-Tubulin	Sigma-Aldrich	Cat# T5168; RRID:AB_477579
HRP-conjugated anti-rabbit	Cell Signaling	Cat# 7074; RRID:AB_2099233
HRP-conjugated anti-mouse	Cell Signaling	Cat# 7076; RRID:AB_330924
IRDye®-conjugated anti-mouse	LiCor	Cat# 925-68070; RRID:AB_2651128
IRDye®-conjugated anti-rabbit	LiCor	Cat# 925-68071; RRID:AB_2721181
mouse anti-Piwi	Santa Cruz	Cat# sc-390946
Rat anti-vasa	DSHB	AB_760351; RRID:AB_760351
Anti-rat Alexa Fluor 594	Invitrogen	Cat# A-11007; RRID:AB_141374
Mouse 6x-His Tag Monoclonal Antibody	ThermoFisher	Cat# His.H8; RRID:AB_2536988
GFP-Trap®	ChromoTek	Cat# gtma-20; RRID:AB_2631358
Critical commercial assays
PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit	Cell Signaling	59322
Deposited data
Mass-spec raw and MaxQuant processed data	PRIDE	PXD037421
Experimental models: Cell lines
Drosophila S2 cells	DGRC	S2-DRSC #181
Experimental models: Organisms/strains
6xHis-smt3[T86R]}attP2	This study	N/A
UASp-shWhite	BDSC	33623
UASp-shSUMO	Ninova et al. 202014,21	N/A
UASp-shPiwi	BDSC	33724
UASp-shSv210-2	BDSC	32956
UASp-GFP-Panx	Rogers et al. 201748	N/A
UASp-GFP-Mael	Webster et al. 201539	N/A
UASp-Spindle-E	Webster et al. 201539	N/A
GFP-Piwi	Le Thomas et al. 201320	N/A
GFP-Aub	Webster et al. 201539	N/A
P{ry[+t7.2] = PZ}Sumo[04493] cn[1]/CyO; ry[506]	BDSC	11378
Iso-1	BDSC	2057
w[∗]; P{w[+mC] = matalpha4-GAL-VP16}V37	BDSC	7063
Oligonucleotides
Su(var)2-10-F	IDT	CCAGCACAGGACGAACAGCCC
Su(var)2-10-R	IDT	CGTGGAACTGGCGACGGCTT
Piwi-F	IDT	CTGCTGATCTCCAAAAATAGGG
Piwi-R	IDT	TCGCGTATAACTGCTCATGG
rp49-F	IDT	CCGCTTCAAGGGACAGTATCTG
rp49-R	IDT	ATCTCGCCGCAGTAAACGC
Recombinant DNA
Drosophila Gateway Vector collection	DGRC	N/A
pActin-3xFlag3xHA-SUMO	Ninova et al. 202014,21	N/A
pUbi-GFP-Piwi	This study	N/A
pUbi-GFP-Panx	This study	N/A
pUbi-GFP-Mael	This study	N/A
pUbi-GFP-SpnE	This study	N/A
Software and algorithms
Rmd notebook	This study	https://doi.org/10.5281/zenodo.7834381
clusterProfiler	Yu et al. 201249	N/A
cytoScape	Shannon et al. 200350	v. 3.9.1
BioEmbeddings	Dallago et al. 202124	N/A
MUSCLE	Edgar, 200451	N/A
GPS-SUMO	Zhao et al., 201452	http://sumosp.biocuckoo.org/
MaxQuant	Cox et al. 200853	v. 1.6.10.43
IUPred2A	Mészáros et al. 201825	N/A
FlyBase		http://flybase.org
Other
Schneider’s Drosophila Medium	Gibco (Life Technologies)	21720–024
Fetal Bovine Serum	GEMINI bio-products	100–106
Penicillin/Streptomycin	Gibco (Life Technologies)	15140–122