Figure 4.

TP53 and telomerase activity

(A) Receiver operating characteristic for TP53 classifier run on stranded FPKM RNA-seq.

(B) Violin and strip plots of TP53 scores plotted by TP53 alteration type (n_activated = 11, n_lost = 100, n_other = 866, Wilcoxon p = 0.92).

(C) Violin and strip plots of TP53 RNA expression plotted by TP53 activation status (n_activated = 11, n_lost = 100, n_other = 866, Wilcoxon p = 0.006).

(D) Boxplots of TP53 and telomerase (EXTEND) scores across cancer groups. TMB status is highlighted in orange (hypermutant) or red (ultra-hypermutant). Boxplot represents 5% (lower whisker), 25% (lower box), 50% (median), 75% (upper box), and 95% (upper whisker) quantiles.

(E) Heatmap of RefSig mutational signatures for patients with at least one hypermutant tumor or cell line.

(F) Forest plot depicting prognostic effects of TP53 and telomerase scores on overall survival (OS), controlling for extent of tumor resection, LGG group, and HGG group.

(G) Forest plot depicting the effect of molecular subtype on HGG OS. Hazard ratios (HRs) with 95% confidence intervals and p values (multivariate Cox) are given in (F) and (G). Black diamonds denote significant p values, and gray diamonds denote reference groups.

(H) Kaplan-Meier curve of HGGs by molecular subtype.