Table 3.
Individual associations between EV-miRNA principal components and technical variables including lab variables and sequencing quality indicators.
| PC1 | PC2 | |||||
|---|---|---|---|---|---|---|
| β (95% CI) | P | PFDR | β (95% CI) | P | PFDR | |
| Lab Variables | ||||||
| Date of EV extraction | 1.97 (-2.68, 6.61) | 0.40 | 0.72 | -0.77 (-4.99, 3.46) | 0.72 | 0.84 |
| Volume of skim milk | -0.02 (-0.04, -0.009) | 0.001 | 0.004 | 0.007 (-0.005, 0.02) | 0.26 | 0.79 |
| Sequencing Quality Indicators | ||||||
| Proportion of rRNA reads | -10.75 (-33.96, 12.46) | 0.36 | 0.72 | -50.79 (-69.79, -31.80) | 6.2x10-7 | 3.1x10-6 |
| Proportion of unmapped reads | 33.65 (25.13, 42.16) | 3.6x10-12 | 2.2x10-11 | 17.34 (8.14, 26.54) | 0.0003 | 0.001 |
| Low transcriptome:genome ratio (Ref = No) | 7.71 (3.38, 12.05) | 0.0006 | 0.003 | 1.70 (-2.48, 5.89) | 0.42 | 0.84 |
Beta coefficients and 95% confidence intervals (CI) from univariable linear regression analysis used to examine the associations between principal components 1 and 2 (PC1 and PC2) and technical variables including lab variables and sequencing quality indicators. P-values in bold denote statistical significance for P or PFDR < 0.05. Skim milk refers to the volume of milk remaining following centrifuge.