TABLE 2.

Mutations in helix α3 and the loop connecting helices α4 and α5 do not affect toxicity

Mutation	Trypsin stabilitya	Toxicityb
Q154R	Unstable
I145D	Stable	No change
A149V	Stable	No change
F148I	Stable	No change
V150D	Stable	No change
I145T	Stable	No change
I145V	Stable	No change
N94V (helix α3)	Stable	No change
N94F (helix α3)	Stable	No change
N105V (helix α3)	Stable	No change
N105F (helix α3)	Stable	No change

^aAs demonstrated in Fig. 1. 

^bLC₅₀s were determined with infected E. coli cells as described in Materials and Methods, with values as reported for the wild type in footnote d of Table 1.