TABLE 1.
Evaluation of the Intra- and Inter-day precision, recovery and matrix effects by the proposed UPLC-MS/MS method for determination of macitentan and ACT-132577 in rat plasma (n = 6).
| Analytes | Preparation concentration (ng/mL | Intra-day | Inter-day | Recovery (%) | Matrix effects (%) | ||
|---|---|---|---|---|---|---|---|
| Precision RSD (%) | Accuracy RE (%) | Precision RSD (%) | Accuracy RE (%) | ||||
| Macitentan | 4 | 6.90 | 10.05 | 9.36 | 8.62 | 86.43 | 86.16 |
| 160 | 5.24 | 6.57 | 2.79 | 7.59 | 95.94 | 92.87 | |
| 1,600 | 4.79 | 9.55 | 3.67 | 7.77 | 90.91 | 91.54 | |
| ACT-132577 | 30 | 7.01 | 11.52 | 6.83 | 8.74 | 88.12 | 89.77 |
| 900 | 4.53 | 8.49 | 5.9 | 5.83 | 90.40 | 94.02 | |
| 9000 | 3.68 | 2.28 | 2.49 | 2.92 | 92.18 | 93.18 | |
Abbreviations: RSD, relative standard division; RE, relative error.