TABLE 2.

Stability evaluation results of macitentan and ACT132577 in rat plasma under different conditions (n = 6).

Analytes	Preparation concentration (ng/mL)	Room temperature, 6 h		4°C, 24 h		−80°C, 7days
		RSD (%)	RE (%)	RSD (%)	RE (%)	RSD (%)	RE (%)
Macitentan	4	4.72	113.51	4.44	107.57	5.33	104.39
	160	7.94	109.43	2.28	104.86	2.34	103.80
	1,600	3.30	105.63	3.47	106.50	2.92	105.44
ACT-132577	30	7.56	109.84	8.71	106.81	6.58	105.76
	900	8.27	106.18	5.60	104.77	3.20	107.26
	9000	8.50	107.44	3.35	105.28	4.67	103.40

Abbreviations: RSD, relative standard division; RE, relative error.