Figure 1.

Saccharomyces boulardii (Sb) alleviated clinical symptoms in DSS-induced colitis. (A) Average body weight. (B) Colon length of mice. (C) Colorectal weight/length ratio of mice. (D) DAI were recorded and calculated every other day. (E) Appearance of H&E stained colon section and histopathological score. (Magnification: ×200; Scale bar: 50 μm). Immunofluorescence analysis and score of TUNEL (green) in colon mucosa (200 × magnification). DAPI was used for nuclear staining (blue). (F) Representative immunofluorescence images and quantification of Occludin and ZO-1, in distal ileum (200 × magnification; Scale bar: 20 μm). DAPI was used for nuclear counterstaining (blue). (G) Serum inflammatory mediators of MPO, HMGB1, TNF-α, IL-6, IL-1β and MCP-1 were determined by ELISA kits. Data are presented as mean ± SEM of 6 mice per group. ^#p < 0.05 compared with the control group; ^*p < 0.05 compared with the DSS group. Sb, S. boulardii.