TABLE 2.
Alkaline phosphatase activities of TraD-PhoA hybrid proteins
| Hybrid proteina | Alkaline phosphatase activityb (SD) | Hybrid protein synthesis ratec (SD) |
|---|---|---|
| pNLK5 | 2.5 (0.62) | NA |
| K6 | 21 (9.9) | 5.0 (2.2) |
| I14 | 11 (4.1) | 2.1 (0.4) |
| I59 | 750 (190) | 0.76 (0.1) |
| T65 | 250 (270) | ND |
| E67 | 160 (5) | 1.0 (0.1) |
| Q94 | 46 (5.0) | 0.73 (0.2) |
| G282 | 5.1 (1.3) | 0.77 |
| E620 | 6.0 (0.65) | 0.65 |
| N702 | 4.9 (2.3) | ND |
a
Encoded by pNLK5 derivatives.
b
Expressed in arbitrary units (24). Values are averages of 3 to 14 assays each.
c
Expressed relative to alkaline phosphatase encoded by the chromosome and expressed constitutively. TraD expression values are the average of one to two assays each. NA, not applicable; ND, not determined.