TABLE 3.
Complementation efficiencies of TraD 31-codon insertion alleles
| traD allele | Transfer efficiencya (transconjugants/donor) |
|---|---|
| Vector (pBAD18) | 7.4 × 10−5 |
| K6 | 9.8 × 10−4 |
| I14 | 1.1 × 10−3 |
| I59 | 1.0 × 10−3 |
| T65 | 0.23 |
| E67 | 0.25 |
| Q94 | 0.74 |
| G282 | 2.6 × 10−4 |
| E620b | 1.5 × 10−3 |
| N702 | 1.5 |
a
Relative to EM1014 carrying the wild-type traD parent plasmid pNLK5, assigned a value of 1. This strain gave ∼3 transconjugants/donor under the conditions used.
b
The 31-codon insertion generates a frameshift mutation (rendering the C-terminal traD sequence out of frame) because 8 rather than 9 bp were duplicated during insertion of ISlacZ/in (not shown).