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. 2023 Jul 10;14:1206255. doi: 10.3389/fpls.2023.1206255

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Copyright © 2023 Vo, Lal, Nattanong, Tabassum, Qureshi, Troiano, Parrella, Kil and Lee

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infectivity of AV1 chimeric constructs in tomato. (A) Visual symptom of two AV1 constructs (In_V1ES: ToLCNDV-In CP in ToLCNDV-ES backbone; ES_V1In: ToLCNDV-ES CP in ToLCNDV-In backbone) at 21 dpi. (B) PCR detection of viral DNA in infected tomatoes. Lane M: marker, Lane +: positive control, lane −: negative control, lane C: mock plant, lane 1–4: inoculated plants. (C) Southern blot hybridization results of In_V1ES chimera clone; the amplicon from PCR detection was used as probe.