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. 2023 Jul 10;14:1206255. doi: 10.3389/fpls.2023.1206255

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Copyright © 2023 Vo, Lal, Nattanong, Tabassum, Qureshi, Troiano, Parrella, Kil and Lee

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infectivity of amino acid 143 position point mutant clones of coat protein of ToLCNDV. (A) Phenotype of point mutant constructs (ES-V1^W143R: the residue tryptophan at amino acid 143 was changed to arginine in ToLCNDV-ES CP and In-V1^R143W: arginine was changed to tryptophan in ToLCNDV-In CP) in tomato. (B, C) Sampling position and PCR results in different tissues, Lane M: positive control, Lane −: negative control, Lane C: mock plant, NL, new leaf; S, stem; R, root. (D) Relative viral titer in different tissues. The bar graph indicate the mean ± standard deviation (n = 3). The statistical comparison was performed with the unpaired t-test: *p <0.05, ns, not significant.