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. 2023 Jul 10;14:1206255. doi: 10.3389/fpls.2023.1206255

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Copyright © 2023 Vo, Lal, Nattanong, Tabassum, Qureshi, Troiano, Parrella, Kil and Lee

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirmation of Y2H for Ring finger protein 44-like and its expression by RT-qPCR (A) Y2H results when each CP is hybridized with Ring finger protein 44-like. CP of each ToLCNDV as bait and Ring finger protein 44-like as prey; pGBKT53 x pADT7-T is positive control, pGBKT53x pADT7-Lam is negative control. Two selection media are DDO (double dropout SD/-Trp/-Leu) and QDO (Quadruple dropout SD/-Trp/-Leu/-His/-Ade). (B) Relative expression change of Ring finger protein 44-like in different inoculated leaves at 21 dpi. The statistical comparison was performed with the unpaired t-test: **p <0.01.