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. 2023 Jul 10;14:1187196. doi: 10.3389/fimmu.2023.1187196

Figure 4.

Figure 4

Induction of GILZ and FKBP5 transcripts by hydrocortisone is reduced in PBMCs from patients. PBMCs were isolated and incubated for 2 hours with vehicle or 150 nM hydrocortisone. mRNA was extracted and levels of Tsc22d3 (GILZ), Fkbp5 and Nr3c1α (GRα) were measured by qRT-PCR. (A, C) The mRNA fold increase was calculated compared to the mean of the AC vehicle group and the paired effect of the hydrocortisone versus vehicle condition was analyzed for the controls and patients. (B, D) Fold increase by hydrocortisone defined by the ratio of the mRNA fold increase of the hydrocortisone condition over the vehicle condition. (E-G, I) Correlation analyses by Spearman test. (H) mRNA fold increase compared to the AC vehicle group. (A-I) Each symbol represents data from an individual. Analysis was by Mann-Whitney U-test or Wilcoxon test for the paired data. Only significant differences are indicated. * p<0.05, *** p<0.001, **** p<0.0001. AC n=20; UM n=19; SM n=24.