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. 2023 Jul 10;14:1187196. doi: 10.3389/fimmu.2023.1187196

Figure 5.

Figure 5

Malaria infection in mice causes reduced corticosterone sensitivity of splenocytes. Splenocytes were isolated from spleens of non-infected control (Con) C57Bl/6J mice and Plasmodium berghei NK65 (PbNK65)-infected C57Bl/6J mice (day 9 post-infection) and stimulated for 2 hours without or with 400 nM of corticosterone (GC). mRNA levels of Tsc22d3 (GILZ) and Fkbp5 were measured by qRT-PCR. (A, C) mRNA fold increase compared to the Con group. (B, D) Fold increase by GC defined by the ratio of the mRNA fold increase of the GC condition over the untreated condition. The data originates from two separate experiments. Each symbol represents data from an individual mouse. Horizontal lines in between data points represent group medians. Analysis was by Mann-Whitney U-test. Horizontal lines with asterisks on top indicate the levels of statistical significance between the indicated groups. Asterisks above individual data sets indicate the levels of statistical significance compared to the unstimulated group. Only significant differences are indicated. *** p<0.001. Con n=7; PbNK65 n=9.