Figure 7.

GC-mediated induction of cAMP and AMP is reduced in PBMCs from patients with malaria. PBMCs were isolated and incubated for 24 hours with [U-¹³C]-glucose tracer and vehicle (VEH) or 150 nM hydrocortisone (GC). Metabolic extracts were analyzed via mass spectrometry. (A) GC-induced Log2 fold changes in abundancies of 47 metabolites were analyzed by PLS-DA. (B) Analysis of the GC versus VEH condition for the relative abundancy of AMP, cAMP and UMP by paired t-test. (C) Log2 fold increase of abundancy by hydrocortisone. Comparison between groups by Mann-Whitney U-test. Each symbol represents data from an individual. Bars in (B) represent the mean, lines in (C) represent the median. Asterisks above individual data sets indicate statistical differences compared to the AC group. Only significant differences are indicated. PLS-DA, Partial Least Squares Method-Discriminant Analysis; VIP, Variable Importance in Projection. AC n=20; UM n=11-12; SM n=20-21. * p<0.05, *** p<0.001.