Figure 5.
C. albicans hyphae growth induces second wave of neutrophils. (A) WT mice were infected with 106 C. albicans labeled with Uvitex 2B, the growth of hyphae in different organs was studied by fluorescence microscope. (B) The percentage of C. albicans with hyphae growing at 4 h post infection. (C) Whole mount confocal images showing the penetration of tissue by candida hyphae. (D) The length of longest hyphae grows with time in kidney. (E) Whole mount confocal images showing the growth of candida hyphae and association with brain resident macrophage. (F) The regression curve of percent within phagocytes versus percent hyphae growth, five dots represent different organs, the lung, brain, spleen, kidney, and liver. Scale bar=10 μm. ***, p<0.001 by student’s t test.