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. 2023 Jul 10;14:1173728. doi: 10.3389/fimmu.2023.1173728

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Copyright © 2023 Kametani, Ito, Ohshima, Manabe, Ohno, Shimizu, Yamada, Katano, Kirigaya, Ito, Matsumoto, Tsuda, Kashiwagi, Goto, Yasuda, Maeki, Tokeshi, Seki, Fukase, Mikami, Ando, Ishimoto and Shiina

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Preparation of Lipo-anti-PD-L1-P4. (A) Protocol for the preparation of Lipo-anti-PD-L1-P4. (B) Representative result of liposome size distribution. Average size: 44 nm; polydispersity index: 0.22; ζ-potential: –20.1 kV; P4 concentration: 78.5 μg/mL. (C) P4 leakage test. DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; DSPE-PEG-Maleimide, N-[(3-maleimide-1-oxopropyl)aminopropyl polyethyleneglycol-carbamyl] distearoylphosphatidyl-ethanolamine; EDTA, ethylenediaminetetraacetic acid; Lipo-anti-PD-L1-P4, liposome-encapsulated anti-programmed death ligand 1 antibody-conjugated P4; P4, progesterone; PBS, phosphate-buffered saline; r.t., room temperature.