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. 2023 Jul 10;14:1173728. doi: 10.3389/fimmu.2023.1173728

Figure 4.

Figure 4

Effect of Lipo-anti-PD-L1-P4 on cell proliferation. PD-1 and PD-L1 expression on T cells (A, B) and (C) cancer cells. (A) Left panel: Percentage of PD-1 and PD-L1 expressing cells in CD3+ T cells. Right panels: PD-1 and PD-L1 mean fluorescence intensities of CD3+ T cells. (B) Left panel: percentage of PD-1 and PD-L1 expressing cells in CD4+ T cells; right panel: percentage of PD-1 and PD-L1 expressing cells in CD8+ T cells. (C) MDA-MB-231, JEG-3, and Karpas 707H cells were cultured in the presence or absence of P4 (open bars) or Lipo-anti-PD-L1-P4 (solid bars). Vertical lines represent the cell number after 3 d of culture. *P < 0.05; **P < 0.01; ***P < 0.001 (one-way analysis of variance or two-sided Student’s t-test). Data are presented as the mean ± standard deviation of four independent experiments. DN, double-negative; DP, double-positive; Lipo-anti-PD-L1-P4 (Lipo-P4), liposome-encapsulated anti-programmed death ligand 1 antibody-conjugated P4; MFI, mean fluorescence intensity; PD-1, programmed death 1; PD-L1, programmed death ligand 1.