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. Author manuscript; available in PMC: 2024 Jul 1.
Published in final edited form as: FASEB J. 2023 Jul;37(7):e23058. doi: 10.1096/fj.202300838R

Figure 6. E2F7 promotes the transcription of Enpp2 in mouse cancer cells.

Figure 6.

(A) B16-F10 melanoma cells were transfected with E2F7 siRNA and immunoblotting was applied to measure the E2F7, ATX, and β-actin protein expression. (B) B16-F10 cells were crosslinked with 4% (V/V) formaldehyde and total protein was extracted for ChIP-qPCR analysis. ChIP primers were used to target the E2F7-RE in the mouse Enpp2 gene. (C) 3C-qPCR was applied to measure the interaction frequency between Anchor-1 (associated with the intronic E2F7-RE2) and forward primers targeting E2F7-RE1 and promoter fragments in B16-F10 cells. The quantitative data are represented as the mean ± SD based on at least three independent experiments. *p < 0.05, **p<0.01, and ***p < 0.001 indicate significant differences.