Table 2. Mutations Introduced in Codon-Optimized ErCry4a.
mutation | forward primer | reverse primer |
---|---|---|
C68A + C73A | GGATCGGCCCTGCTTGTGATTCAGGGTG | AAGCTGGGCCAGGTTCTTATGCAGATCC |
C116A | GAACATTCAGGCCTTGGGTGCAG | GCCTCCATCTCCTTGTAG |
C189A | TCTTGCCGAAGCCTATCGTGTTCCGTTAC | TCCGGATCTGGAGCCGAA |
C317A | TCTGCAGATTGCCTGGTATAAAGACGCAGAAC | CAGATCGGATTGCCCGCC |
C412A | CCGGATTTTCGCCCCTGTACGCTTTG | GTGTACTGGTGAAAGAAGG |