TABLE 1.

Optimization of PCR primers.

	Observed					Expected
Johnson ID	clbN (733 bp)	cdtB (466 bp)	clbB (579 bp)	cnf1 (974 bp)	uidA (503 bp)	clbN (733 bp)	cdtB (466 bp)	clbB (579 bp)	cnf1 (974 bp)	uidA (503 bp)
1	+		+	+	+	+		+	+	+
2	+		+	+	+	+		+	+	+
3	+		+	+	+	+		+	+	+
4	+	+	+		+	+	+	+		+
5					+					+
6					+					+
7					+					+
8	+		+		+	+		+		+
9					+					+

Note: Escherichia coli strains with listed genotypes, and results, were used to construct the multiplex assay. Strains were courtesy of Dr. James Johnson at the University of Minnesota.

Abbreviation: PCR, polymerase chain reaction.