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. Author manuscript; available in PMC: 2023 Jul 24.
Published in final edited form as: Nat Protoc. 2023 Feb 8;18(4):1296–1315. doi: 10.1038/s41596-023-00803-0

Table 2.

Pipetting scheme to assess the trueness of the DL-2-HG EISA method

Spiked concentration (μmol/L) per DL-2-HG enantiomer
0 (non-spiked) 10 50 70
Concentration (μmol/L) of the stock solutions used for spikinga - 2 2 2
Volume (μL) of stock solution addedb - 1 5 7
Volume (μL) of 1 mmol/L IS solution addeda,b 2 2 2 2
Volume (μL) of methanol added 90 90 90 90
a

The concentration depicted here is the sum of both enantiomers.

b

In situations where volumes lower than 20 μL cannot be accurately taken, use less concentrated stock solutions so that larger volumes of the corresponding aliquots can be used.