Table 4.
Results of assessing the trueness, precision and recovery of the different biological samples by the developed EISA-TOF method
| K562 cells | Urine | FCS | Serum | |||||
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| L-2-HG | D-2-HG | L-2-HG | D-2-HG | L-2-HG | D-2-HG | L-2-HG | D-2-HG | |
| Trueness | % Mean recovery ± SD | |||||||
|
| ||||||||
| 10 μmol/L | 97 ± 4 | 90 ± 5 | 98 ± 8 | 105 ± 5 | 105 ± 8 | 101± 4 | 103 ± 2 | 106 ± 6 |
| 50 μmol/L | 95 ± 2 | 87 ± 2 | 100 ± 8 | 113 ± 10 | 100 ± 5 | 96.7 ± 0.3 | 106 ± 3 | 105 ± 5 |
| 70 μmol/L | 97 ± 2 | 83 ± 5 | 98 ± 3 | 105 ± 5 | 99 ± 2 | 96 ± 3 | 105 ± 1 | 105 ± 2 |
| Precision | RSD (%) | |||||||
|
| ||||||||
| Between-injection repeatability (n=3)a | 5.0 | 5.0 | 3.6 | 3.9 | 1.1 | 2.2 | 1.3 | 2.2 |
| Measurement repeatability (n=3)b | 4.8 | 4.8 | 1.8 | 2.4 | 5.3 | 6.8 | 3.5 | 3.1 |
| Intermediate measurement precision (n=9)c | 4.9 | 4.9 | 4.6 | 4.4 | 6.7 | 7.3 | 3.8 | 5.2 |
| Recovery (before and after the extraction) | % Mean recovery ± SD | |||||||
|
| ||||||||
| 104 ± 8 | 104 ± 8 | 99 ± 2 | 94 ± 5 | 99 ± 3 | 94 ± 7 | 95 ± 3 | 99 ± 5 | |
a
Three consecutive injections of the corresponding biological sample spiked with 10 μmol/L of L-2-HG, 10 μmol/L of D-2-HG, 10 μmol/L of L-2-HG-d3 and 10 μmol/L of D-2-HG-d3.
b
Three replicates of the corresponding biological sample spiked with 10 μmol/L of L-2-HG, 10 μmol/L of D-2-HG, 10 μmol/L of L-2-HG-d3 and 10 μmol/L of D-2-HG-d3 injected in triplicate on the same day.
c
Three replicates of the corresponding biological sample spiked with 10 μmol/L of L-2-HG, 10 μmol/L of D-2-HG, 10 μmol/L of L-2-HG-d3 and 10 μmol/L of D-2-HG-d3 injected during three consecutive days.