TABLE 2.

Relative effects of pilE mutations in transformation and pilE variation

Strain	Nature of pilE	% Transformationa	% pilE variationb
BHAC5	Wild type	100	100
BHAcat3	cys2::′cat	5.6	3.8
BHANot4	cys2::NotI	7.7	6.7
BHAcat5	HVL::′cat	0.42	0.20
BHANot5	HVL::NotI	100	100
BHA-HV	ΔHVL	2.0	1.9

^aThe level of transformation detected in the wild-type control strain BHAC5 was assigned a value of 100%. The percent transformation in each pilE mutant strain relative to the level of transformation in BHAC5 was determined by the following formula: (the frequency of transformation in pilE mutant strain/frequency of transformation in BHAC5) × 100. 

^bThe level of pilE variation detected in the wild-type control strain BHAC5 was assigned a value of 100%. The percentage of pilE variation in each pilE mutant strain relative to the level of variation in the control was determined by the following formula: [fraction of relative transformation of pilE mutant × (fraction of pilE variants among pilE mutant transformants/fraction of pilE variants among BHAC5 transformants)] × 100.