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. 2023 Jun 22;16(8):1671–1689. doi: 10.1111/1751-7915.14296

FIGURE 1.

FIGURE 1

The expression of the ipdc gene of Serratia plymuthica A153 is growth phase‐dependent and controlled by TyrR. (A) Transcription of the ipdc (P ipdc ::lacZ; pMAMV302) promoter fusion throughout growth of S. plymuthica A153. β‐galactosidase activity (open symbols) and growth curves (filled symbols) were determined in LB medium at 30°C in the absence and presence of different concentrations of L‐Tryptophan (L‐Trp). Data are the mean and standard deviation of three biological replicates. Arrow, time point when samples for RNA‐seq were taken. Wt, wild‐type; OD600, optical density at 600 nm. (B) Transcription of the ipdc (P ipdc ::lacZ; pMAMV302) promoter fusion throughout growth in LB medium supplemented with 1 mg/mL L‐Trp at 30°C in different S. plymuthica A153 strains. *p < 0.01, Student's t‐test of tyrR mutant with respect to the A153 wild‐type (wt) strain. No growth defect was observed for the tyrR deficient strain under these conditions.